Mammal mitogenomics from invertebrate‐derived <scp>DNA</scp>

The metabarcoding of vertebrate DNA found in invertebrate-derived (iDNA) has proven a powerful tool for monitoring biodiversity. To date, iDNA primarily been used to detect the presence/absence particular taxa using metabarcoding, though recent efforts demonstrated potential utility these data estimating relative animal abundance. Here, we test whether can also be reconstruct complete mammalian mitogenomes and therefore bring field closer population-level analyses. Specifically, mitogenomic hybridization capture coupled with high-throughput sequencing analyze individual (N = 7) or pooled 5) fly-derived extracts, 1) leech-derived which were known priori contain primate DNA. All sources showed their ability generate large amounts information deeper libraries is predicted allow even more recovery from most samples (90%). Sixty percent extracts allowed (near) mitochondrial genomes (hereafter mitogenomes) that proved useable phylogenomic These findings contribute paving way iDNA-based population studies terrestrial mammals.